Figure 2. High-dose MML-ADV immunization... | Rockefeller University Press

Figure 2.

$Figure 2. High-dose MML-ADV immunization elicits IL-10–producing CD4+ T cells. MML-specific cytokine production by splenic CD4+ T cells 10 or 28 d after i.m. vaccination of mice with high- (1010 vp) or low- (107 vp) dose MML-ADV. (A–C) Multiparameter flow cytometry was used to quantify the total frequency of IFN-γ–, IL-2–, TNF-, or IL-10–producing CD4+ T cells (A), the frequency of cells expressing each of the fifteen possible combinations of IFN-γ, IL-2, TNF, or IL-10 (B) and the fraction of the total response represented by each of the fifteen individual cytokine populations (C). For pie charts, multifunctional Th1 cells are depicted in red and the blue arc highlights IL-10–producing cells. (D) The IFN-γ MFI of CD4+ T cells from mice vaccinated with high-dose MML-ADV (i.m. or s.c.) producing combinations of IFN-γ, TNF, or IL-10 10 d after vaccination. Bars show the median response from individual (n = 3–6) mice (dots; *, P < 0.05) for one of at least three experiments.$

High-dose MML-ADV immunization elicits IL-10–producing CD4⁺ T cells. MML-specific cytokine production by splenic CD4⁺ T cells 10 or 28 d after i.m. vaccination of mice with high- (10¹⁰ vp) or low- (10⁷ vp) dose MML-ADV. (A–C) Multiparameter flow cytometry was used to quantify the total frequency of IFN-γ–, IL-2–, TNF-, or IL-10–producing CD4⁺ T cells (A), the frequency of cells expressing each of the fifteen possible combinations of IFN-γ, IL-2, TNF, or IL-10 (B) and the fraction of the total response represented by each of the fifteen individual cytokine populations (C). For pie charts, multifunctional Th1 cells are depicted in red and the blue arc highlights IL-10–producing cells. (D) The IFN-γ MFI of CD4⁺ T cells from mice vaccinated with high-dose MML-ADV (i.m. or s.c.) producing combinations of IFN-γ, TNF, or IL-10 10 d after vaccination. Bars show the median response from individual (n = 3–6) mice (dots; *, P < 0.05) for one of at least three experiments.