OT-1 cells affect sterilizing immunity versus SIINFEKL-B16 cells in collagen-fibrin gels. Collagen-fibrin gels containing 5 × 105 SIINFEKL-B16 cells/ml of gel alone (♦) or with 106 (■) or 107 (▴)/ml OT-1 cells were incubated at 37°C for up to 5 d. Gels were lysed on the day indicated and assayed for viable B16 cells as described in Materials and methods. Data shown represent mean ± SEM of n = 3 experiments performed in duplicate. Collagen-fibrin gels containing 2 × 107 SIINFEKL-B16 cells/ml of gel in medium containing 20 U IL-2/ml of gel alone (○) or with 107 (•)/ml OT-1cells were incubated at 37°C for up to 7 d, lysed, and assayed for viable B16 cells as described in Materials and methods. Shown are the results of a representative experiment performed in duplicate.
