![Figure 7. IL-10R signaling requirement for IL-10 T reg cell–mediated suppression of DC function. Tg4 and Tg4 IL-10−/− mice were treated with a minimum of 10 i.n. doses of MBP Ac1-9[4Y]. Splenic DCs were isolated from untreated B10.PL mouse spleens and 5 × 104 DCs were cultured either alone or with equal numbers of CD4+ T cells positively selected from untreated or peptide-treated Tg4 or Tg4 IL-10−/− mice. 100 µg/ml of Ac1-9[4K] and/or 10 µg/ml of anti–IL-10R or isotype control antibody were added where indicated. (A) After 24 h, DCs were stained for surface CD11c, CD80, CD86, and CD40. Results are depicted histograms for each surface protein after gating on CD11c+ cells, with x and y axes showing the fluorescence intensity and percentage of max, respectively. (B) Supernatant cultures described in A were analyzed for IL-12 by ELISA at 24 h after in vitro restimulation. Results are depicted as the mean IL-12 production in nanogram/milliliter of supernatant ± SEM. Data are representative of at least two individual experiments.](https://cdn.rupress.org/rup/content_public/journal/jem/206/8/10.1084_jem.20082118/6/jem_20082118_lw_fig7.jpeg?Expires=1768786015&Signature=yRiLaJkb6RA1c8sUQ9zTKifi-Acqo6godo44dH4qkN3eAol6ZRS2EXJJmU4zps-HDhTiaPefUc2rgRgbc3qT9xN~9Rijc5y3HWHN~qFloxSAob9vEMH65R7YVUpUTRapII1Eozj9u3cPbsZPNbtFPenJq0RT~f3GcN-IFttl-RlFlEkhiyAyG4LRK4nRciTBn5lfuzDMNo6QriU7YnEKQw8-XI6ihHicH6ys01SYUintIVfmYfBafXdPVOkt~NULC-xmtk6QxEuO~XvSJQWeQwVesGClL8SxNEHFbb0ICv03avzB7RDQyyW~xNCOV-VRk7~zMxhaqJ22eSZycv2yYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-10R signaling requirement for IL-10 T reg cell–mediated suppression of DC function. Tg4 and Tg4 IL-10−/− mice were treated with a minimum of 10 i.n. doses of MBP Ac1-9[4Y]. Splenic DCs were isolated from untreated B10.PL mouse spleens and 5 × 104 DCs were cultured either alone or with equal numbers of CD4+ T cells positively selected from untreated or peptide-treated Tg4 or Tg4 IL-10−/− mice. 100 µg/ml of Ac1-9[4K] and/or 10 µg/ml of anti–IL-10R or isotype control antibody were added where indicated. (A) After 24 h, DCs were stained for surface CD11c, CD80, CD86, and CD40. Results are depicted histograms for each surface protein after gating on CD11c+ cells, with x and y axes showing the fluorescence intensity and percentage of max, respectively. (B) Supernatant cultures described in A were analyzed for IL-12 by ELISA at 24 h after in vitro restimulation. Results are depicted as the mean IL-12 production in nanogram/milliliter of supernatant ± SEM. Data are representative of at least two individual experiments.
