Pulmonary pDCs and CD8α⁺ DCs express IL-15 and IL-15Rα. (A) Groups of BALB/c mice were infected with a sublethal dose of IAV. On day 6 p.i., lungs were pooled and pDCs, CD8α⁺ DCs, and aMϕs were FACS purified and analyzed by qRT-PCR analysis for IL-15 expression. Results were normalized to GAPDH expression. Data are pooled from three separate experiments and represent means ± SEM. (B) Groups of BALB/c mice were infected with a sublethal dose of IAV. On day 6 p.i., pDCs, CD8α⁺ DCs, and aMϕs in the lungs were analyzed for surface expression of IL-15Rα (continuous line) versus isotype control (shaded) by flow cytometry. Data are representative of three separate experiments.