Figure 5.
Figure 5. DCs are capable of maintaining iBALT as a result of chemokine expression. (A) Relative expression of lymphotoxin-β, CXCL12, CXCL13, CCL19, and CCL21 on sorted CD11b+ DCs obtained from pooled lung tissues of 10 infected mice at various dpi. Bars indicate relative expression. (B) Relative expression of lymphotoxin-β, CXCL13, and CCL21 on total lung RNA of naive mice and with or without DT treatment. Bars represent mean values of five mice per group with error bars indicating SEM. *, P < 0.05; ns, not significant. (C) Mice infected at day 0 were treated with LTβR-Fc or an isotype at 17 dpi and analyzed 24 dpi. The left histogram represents GL-7–positive cells in lung tissue measured by flow cytometry, with bars representing mean values of five mice per group and error bars indicating SEM. *, P < 0.05. Histology of spleen (top) and lung (bottom) demonstrate high-power field images of GL7-IgD aggregates after treatment with LTβR-Fc or an isotype, representative of at least five mice per group. Bars, 1,000 µm. The right histogram demonstrates numbers of counted iBALT/hight-power field. Bars represent mean values of three high-power fields counted per lung of at least five animals per group. Error bars indicate SEM. **, P < 0.01. (D) Relative expression of lymphotoxin-β, CXCL13, and CCL21 on total lung RNA of naive mice and 1 wk after LTβR-Fc or isotype treatment. Bars represent mean values of five mice per group with error bars indicating SEM. *, P < 0.05; **, P < 0.005; ns, not significant. (E) Repetitive adoptive transfer of cultured GM-CSF DCs induced iBALT. DCs were grown from BM and injected five times with 2-wk intervals as a control PBS was injected. Consecutive histological slides show presence of iBALT aggregates. Bars, 100 µm. The histogram demonstrates numbers of counted iBALT per high-power field. Bars represent mean values of three low-power fields counted per lung of at least five animals per group. Error bars indicate SEM. *, P < 0.05. All experiments in this figure have been independently performed twice with at least five mice per group.

DCs are capable of maintaining iBALT as a result of chemokine expression. (A) Relative expression of lymphotoxin-β, CXCL12, CXCL13, CCL19, and CCL21 on sorted CD11b+ DCs obtained from pooled lung tissues of 10 infected mice at various dpi. Bars indicate relative expression. (B) Relative expression of lymphotoxin-β, CXCL13, and CCL21 on total lung RNA of naive mice and with or without DT treatment. Bars represent mean values of five mice per group with error bars indicating SEM. *, P < 0.05; ns, not significant. (C) Mice infected at day 0 were treated with LTβR-Fc or an isotype at 17 dpi and analyzed 24 dpi. The left histogram represents GL-7–positive cells in lung tissue measured by flow cytometry, with bars representing mean values of five mice per group and error bars indicating SEM. *, P < 0.05. Histology of spleen (top) and lung (bottom) demonstrate high-power field images of GL7-IgD aggregates after treatment with LTβR-Fc or an isotype, representative of at least five mice per group. Bars, 1,000 µm. The right histogram demonstrates numbers of counted iBALT/hight-power field. Bars represent mean values of three high-power fields counted per lung of at least five animals per group. Error bars indicate SEM. **, P < 0.01. (D) Relative expression of lymphotoxin-β, CXCL13, and CCL21 on total lung RNA of naive mice and 1 wk after LTβR-Fc or isotype treatment. Bars represent mean values of five mice per group with error bars indicating SEM. *, P < 0.05; **, P < 0.005; ns, not significant. (E) Repetitive adoptive transfer of cultured GM-CSF DCs induced iBALT. DCs were grown from BM and injected five times with 2-wk intervals as a control PBS was injected. Consecutive histological slides show presence of iBALT aggregates. Bars, 100 µm. The histogram demonstrates numbers of counted iBALT per high-power field. Bars represent mean values of three low-power fields counted per lung of at least five animals per group. Error bars indicate SEM. *, P < 0.05. All experiments in this figure have been independently performed twice with at least five mice per group.

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