Figure 4.
Figure 4. ConA promotes stronger liver damage in NEMOΔhepa mice than in NEMOf/f animals. (A–C) ALT serum levels (A), H&E staining and TUNEL analysis (B), and quantification of caspase 8 and 3 activity (C) measured 6 h after 25 mg/kg ConA showed strong liver damage in NEMOΔhepa mice. (D) mRNA levels of IFN-γ, IL-4, CCL5, IL-12p35, TNF, and IL-10. All RT-PCR data are presented as times versus NEMOf/f untreated. (E) ELISA of IFN-γ and TNF in serum before and 6 h after 25 mg/kg ConA. Bars, 50 µm. All data are representative of three independent experiments. n = 4. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (NEMOf/f vs. NEMOΔhepa). §§, P < 0.01; §§§, P < 0.001 (NEMOΔhepa vs. ConA/NEMOΔhepa). Error bars represent SD.

ConA promotes stronger liver damage in NEMOΔhepa mice than in NEMOf/f animals. (A–C) ALT serum levels (A), H&E staining and TUNEL analysis (B), and quantification of caspase 8 and 3 activity (C) measured 6 h after 25 mg/kg ConA showed strong liver damage in NEMOΔhepa mice. (D) mRNA levels of IFN-γ, IL-4, CCL5, IL-12p35, TNF, and IL-10. All RT-PCR data are presented as times versus NEMOf/f untreated. (E) ELISA of IFN-γ and TNF in serum before and 6 h after 25 mg/kg ConA. Bars, 50 µm. All data are representative of three independent experiments. n = 4. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (NEMOf/f vs. NEMOΔhepa). §§, P < 0.01; §§§, P < 0.001 (NEMOΔhepa vs. ConA/NEMOΔhepa). Error bars represent SD.

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