Figure 2. Ablation of lymph S1P by conditional deletion of Sphk1 in Sphk2-deficient mice. (A) Flow cytometric analysis showing S1P1 on CD4+ CD62Lhi T cells from the indicated circulatory fluids and tissues. Δ indicates Lyve-1 Cre+ Sphk1f/− or f/f Sphk2−/− mice; C indicates littermate control. The shaded histograms show staining with control antibody of cells from LN and blood. Bld, blood; Lym, lymph; Spl, spleen. (B) Measurement of S1P level by bioassay. Lymph fluid and plasma samples were prepared (see Materials and methods) and titrated onto WEHI231 cells expressing FLAG-S1P1. The x axis shows dilution of the samples. The y axis indicates mean fluorescence intensity (MFI) of FLAG antibody staining. Data are representative of at least five experiments with one to two mice each.
Figure 2.

Ablation of lymph S1P by conditional deletion of Sphk1 in Sphk2-deficient mice. (A) Flow cytometric analysis showing S1P1 on CD4+ CD62Lhi T cells from the indicated circulatory fluids and tissues. Δ indicates Lyve-1 Cre+ Sphk1f/− or f/f Sphk2−/− mice; C indicates littermate control. The shaded histograms show staining with control antibody of cells from LN and blood. Bld, blood; Lym, lymph; Spl, spleen. (B) Measurement of S1P level by bioassay. Lymph fluid and plasma samples were prepared (see Materials and methods) and titrated onto WEHI231 cells expressing FLAG-S1P1. The x axis shows dilution of the samples. The y axis indicates mean fluorescence intensity (MFI) of FLAG antibody staining. Data are representative of at least five experiments with one to two mice each.

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