Figure 1.
EBER1 is released into the culture supernatants of EBV-infected Mutu+, Akata+ and LCL. (A) RT-PCR of EBER1. Cells (2 × 105 cells/ml) were cultured for the designated number of days. Total RNA was extracted from 1 ml culture supernatant and subjected to 25 cycles of RT-PCR to detect EBER1. Three or more independent experiments were performed. (B) Quantitative RT-PCR of EBER1 and EBER2. Cells (2 × 105) were cultured in 1 ml medium for 4 d, and total RNA was extracted from the culture supernatant and subjected to RT-RCR for the detection of EBER1 and EBER2. Error bars indicate the SD of duplicate wells. The data presented are representative of three independent experiments.
