Figure 4.
Figure 4. RARα deficiency but not RARα1 deficiency blocks T reg cell conversion. (A) Naive CD4+ T cells were activated with anti-CD3 and anti-CD28 for 3 d under the indicated conditions, and T reg cell or Th17 conversion was analyzed by intracellular staining of Foxp3 or IL-17 (percentages are indicated). Representative FACS plots are shown of three independent experiments. (B) IL-6 reduces the RARα content of naive T cells. Naive CD4+ T cells were activated as in A. After 24 h, cells were collected for the determination of RARα by TaqMan quantitative PCR. Data are representative of three independent experiments.

RARα deficiency but not RARα1 deficiency blocks T reg cell conversion. (A) Naive CD4+ T cells were activated with anti-CD3 and anti-CD28 for 3 d under the indicated conditions, and T reg cell or Th17 conversion was analyzed by intracellular staining of Foxp3 or IL-17 (percentages are indicated). Representative FACS plots are shown of three independent experiments. (B) IL-6 reduces the RARα content of naive T cells. Naive CD4+ T cells were activated as in A. After 24 h, cells were collected for the determination of RARα by TaqMan quantitative PCR. Data are representative of three independent experiments.

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