Figure 3. Deletion of Ift20 leads to mitotic spindle misorientation in p5 collecting duct cells. (A) In normal kidney tubules, mitotic spindles (phospho-histone H3, red) typically orient their axes (white lines) parallel to the long axis of the collecting duct (DBA, green; left panels in A, gray bars in B). The absence of IFT20 disrupts this arrangement, producing a more random orientation of mitotic spindles (right panels in A, black bars in B). Images are maximum projections of 16 confocal z images taken 0.5 μm apart. Bar, 5 μm. (B) Mitotic spindle orientation quantitation. Mitotic collecting duct cells were photographed, and the angle between the long axis of the tubule and the spindle was measured; angles were grouped into 10° bins. Bars in the inset show circular mean mitotic spindle orientation and the 95% confidence interval about the mean. ***, P < 0.001 (Kolmogorov-Smirnov test).
Figure 3.

Deletion of Ift20 leads to mitotic spindle misorientation in p5 collecting duct cells. (A) In normal kidney tubules, mitotic spindles (phospho-histone H3, red) typically orient their axes (white lines) parallel to the long axis of the collecting duct (DBA, green; left panels in A, gray bars in B). The absence of IFT20 disrupts this arrangement, producing a more random orientation of mitotic spindles (right panels in A, black bars in B). Images are maximum projections of 16 confocal z images taken 0.5 μm apart. Bar, 5 μm. (B) Mitotic spindle orientation quantitation. Mitotic collecting duct cells were photographed, and the angle between the long axis of the tubule and the spindle was measured; angles were grouped into 10° bins. Bars in the inset show circular mean mitotic spindle orientation and the 95% confidence interval about the mean. ***, P < 0.001 (Kolmogorov-Smirnov test).

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