Figure 2.
Relationship between cilia loss and cystic expansion. Kidneys from control and experimental animals were stained for cilia (611β1, green), collecting ducts (aquaporin-2, red), and nuclei (DAPI, blue) at e18, which is the day before birth, and p5, p10, and p23. Arrows indicate collecting ducts and arrowheads indicate ciliated nephrons near collecting ducts; cysts and dilated ducts are indicated by a “C.” Images are maximum projections of 16 confocal z images taken 0.5 μm apart. Bar, 10 μm.