Cytokine production by dermal DC and macrophages. Cytokine analysis in the supernatant from 24-h culture of dermal DC, macrophages, and blood monocytes. 40,000 sorted cells were left unstimulated or treated with 10 ng/ml peptidoglycan (PGN), 10 µg/ml poly IC, and 0.1 µg/ml LPS. Data shown are the mean ± SEM of at least three independent experiments.