Regulation of BRP-39 during antigen-induced Th2 inflammation. (A–F) WT BALB/c mice were sensitized with OVA plus alum and challenged with PBS or OVA (A–E) or sensitized with HDM antigen plus alum and challenged with PBS or HDM (F). (A) The levels of BRP-39 mRNA were evaluated by real time RT-PCR. (B and C) BRP-39 accumulation was assessed via Western analysis (B) and ELISA (C) using lung lysate and BAL fluid, respectively. (D) Immunohistochemistry (IHC) was also used to localize the BRP-39 (solid arrow, airway epithelial cell; open arrow, alveolar macrophage). (E) Double-label IHC using BRP-39 and F4/80 antibodies, localized BRP-39, and F4/80-positive macrophages (white arrows, double⁺ cells). (F) The levels of BRP-39 in BAL fluids from PBS- or HDM-challenged mice, assessed by ELISA. Values in A, C, and F are the mean ± SEM of evaluations in a minimum of five animals and are representative of two separate evaluations. B, D, and E are illustrative of a minimum of three separate experiments. **, P < 0.01. Bars, 25 µm.