TSLP–TSLR interactions limit proinflammatory cytokine production and infection-induced inflammation. (A–C) TSLPR^−/− mice have increased infection-induced inflammation. (A) Paraffin-embedded cecal sections from day 34 after infection were stained with H&E. (B) Epithelial cells in TSLPR^−/− mice exhibit numerous mitotic figures (arrowheads). (C) TSLPR^−/− mice exhibit foci of inflammation with loss of crypt architecture. (D–F) TSLPR^−/− mice have increased proinflammatory cytokine production at day 20 after infection. (D) Frequencies of CD4⁺ IFN-γ⁺ T cells in the MLNs at day 20 after infection (percentages are shown). (E) Antigen-specific IFN-γ production from restimulated MLNs was determined by ELISA. (F) Polyclonal IL-17A production from restimulated MLNs was determined by ELISA. Results represent means ± SEM. Data represent two to three individual experiments with three to four mice per group. *, P ≤ 0.05. Bars, 50 µm.