Figure 3.
Figure 3. TSLP responsiveness is required for immunity to Trichuris. (A) TSLPR−/− mice are unable to induce Th2 cytokine production. Antigen-specific cytokine production from restimulated MLN cells was determined by ELISA. (B–D) TSLPR−/− mice have decreased goblet cell responses. ND, not detected. (B) Cecal sections from TSLPR+/+ or TSLPR−/− mice were taken at day 20 after infection and stained with PAS/Alcian blue. (C) Paraffin sections of cecal tissue were IF stained for RELMβ and GOB5 (green) and costained with DAPI (blue). (D) Protein isolated from fecal pellets on various days after infection was analyzed by SDS-PAGE and immunoblotted for RELMβ. N, naive, uninfected control. (E) Worm burdens in TSLPR+/+ and TSLPR−/− mice were assessed at days 20 and 34 after infection. Results represent means ± SEM. Data represent three individual experiments with three to four mice per group. *, P ≤ 0.05. Bars, 50 µm.

TSLP responsiveness is required for immunity to Trichuris. (A) TSLPR−/− mice are unable to induce Th2 cytokine production. Antigen-specific cytokine production from restimulated MLN cells was determined by ELISA. (B–D) TSLPR−/− mice have decreased goblet cell responses. ND, not detected. (B) Cecal sections from TSLPR+/+ or TSLPR−/− mice were taken at day 20 after infection and stained with PAS/Alcian blue. (C) Paraffin sections of cecal tissue were IF stained for RELMβ and GOB5 (green) and costained with DAPI (blue). (D) Protein isolated from fecal pellets on various days after infection was analyzed by SDS-PAGE and immunoblotted for RELMβ. N, naive, uninfected control. (E) Worm burdens in TSLPR+/+ and TSLPR−/− mice were assessed at days 20 and 34 after infection. Results represent means ± SEM. Data represent three individual experiments with three to four mice per group. *, P ≤ 0.05. Bars, 50 µm.

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