In vivo neutralization of TSLP in WT mice results in susceptibility to Trichuris. (A) Antigen-specific cytokine production from restimulated MLN cells was determined by ELISA. (B) Frequencies of CD4⁺ IL-13⁺ T cells in the MLNs after 72 h of antigen restimulation (percentages are shown). Plots are gated on CD4⁺ T cells. (C–E) Anti-TSLP–treated mice fail to induce goblet cell responses after infection. (C) Paraffin sections of cecal tissue were stained with PAS/Alcian blue for goblet cell visualization. (D) Paraffin sections of cecal tissue were IF stained for RELMβ and GOB5 (green) and costained with DAPI (blue). (E) Protein isolated from fecal pellets on various days after infection was analyzed by SDS-PAGE and immunoblotted for RELMβ. N, naive, uninfected control. (F) Worm burdens were assessed at day 21 after infection. Results represent means ± SEM. Data represent three individual experiments with three to six mice per group. *, P ≤ 0.05. Bars, 50 µm.