TSLP is basally expressed in the large intestine. (A) RNA was isolated from sections of the intestine and expression of TSLP was analyzed by RT-PCR. Values represent the percentage of actin expression in each sample. Results represent means ± SEM. Ce, cecum; dis, distal; DSI, distal small intestine; prox, proximal. (B) IF staining of TSLP (red) in intestinal cryosections, costained with DAPI (blue). Prox LI, proximal large intestine. Data represent two individual experiments with two to three mice per group. Bars, 50 µm.