Figure 2. Endogenous Gfi-1 induced by IL-4 suppresses IL-17 production. (A and B) CD4+ T cells from naive mice were activated under Th2 conditions for 4 d. After 2 d, cultured in complete RPMI 1640 with IL-2, cells were either (A) unsorted or (B) sorted for GFP+ (IL-4–producing) cells and were further stimulated under Th2 or Th17 conditions for another 4 d. (C) CD4+ T cells were first activated under Th17 conditions before they were stimulated under Th2 or Th17 conditions for the second round. Cells were counted before and after the second round of stimulation. Fold cell expansion was calculated. Cytokine production was assessed by intracellular staining after PMA plus ionomycin stimulation. Numbers indicate the percentage of cells in each quadrant or gate. Error bars represent means ± SD, and data are representative of two independent experiments.
Figure 2.

Endogenous Gfi-1 induced by IL-4 suppresses IL-17 production. (A and B) CD4+ T cells from naive mice were activated under Th2 conditions for 4 d. After 2 d, cultured in complete RPMI 1640 with IL-2, cells were either (A) unsorted or (B) sorted for GFP+ (IL-4–producing) cells and were further stimulated under Th2 or Th17 conditions for another 4 d. (C) CD4+ T cells were first activated under Th17 conditions before they were stimulated under Th2 or Th17 conditions for the second round. Cells were counted before and after the second round of stimulation. Fold cell expansion was calculated. Cytokine production was assessed by intracellular staining after PMA plus ionomycin stimulation. Numbers indicate the percentage of cells in each quadrant or gate. Error bars represent means ± SD, and data are representative of two independent experiments.

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