Ablation of adult terminal SCs in the presence of EtHD. (A) A pseudocolored adult NMJ with three terminal SCs (white, magenta, and cyan). (B) Time-lapse recording of area boxed in A over a period of 5 h showing a two-photon laser–induced ablation of the magenta-colored terminal SC in the presence of EtHD (orange arrowhead indicates the site of EtHD influx). After ablation of the magenta SC, the cyan cell was photobleached (cyan arrowhead). Note the absence of EtHD influx in the bleached terminal SC and the expansion (white arrowheads) of the unbleached (white) terminal SC. (C and D) Confocal view of the same NMJ after fixation and counterstaining with BTX (C) and DAPI (D). The nucleus of the ablated terminal SC is filled with EtHD (orange arrowheads in C and D), whereas the bleached terminal SC is not (cyan arrowheads in C and D). (E) Higher magnification of boxed area in D. The timers shown represent hours/minutes. Bars, 5 µm.