Figure 3. Chronic in vivo imaging of mature terminal SCs. (A and B) In vivo images of the same NMJs in the sternomastoid muscle of living SC-GFP mice obtained several weeks apart (interval 2.2 ± 1.0 mo; age at onset of imaging: 4.7 ± 1.1 mo, n = 53 adult NMJs, 10 mice). AChRs were labeled with a nonblocking concentration of BTX (red). Axons are labeled with thy1-CFP (green). (A) Addition of terminal SCs. NMJ with two terminal SCs at 6.5 mo and four terminal SCs at 10 mo. (B) Territory reconstruction of individual terminal SCs from A based on photobleaching in the living animal (bleach 1–3) reveals two new terminal SCs with substantial synaptic territory. (C) Translocation of terminal SCs. NMJ at 2.5 mo with five terminal SCs, one of which translocates across the synapse during the following 3.5 mo (orange arrowheads). Bars, 5 µm.
Figure 3.

Chronic in vivo imaging of mature terminal SCs. (A and B) In vivo images of the same NMJs in the sternomastoid muscle of living SC-GFP mice obtained several weeks apart (interval 2.2 ± 1.0 mo; age at onset of imaging: 4.7 ± 1.1 mo, n = 53 adult NMJs, 10 mice). AChRs were labeled with a nonblocking concentration of BTX (red). Axons are labeled with thy1-CFP (green). (A) Addition of terminal SCs. NMJ with two terminal SCs at 6.5 mo and four terminal SCs at 10 mo. (B) Territory reconstruction of individual terminal SCs from A based on photobleaching in the living animal (bleach 1–3) reveals two new terminal SCs with substantial synaptic territory. (C) Translocation of terminal SCs. NMJ at 2.5 mo with five terminal SCs, one of which translocates across the synapse during the following 3.5 mo (orange arrowheads). Bars, 5 µm.

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