Figure 3. MitOS interacts with both outer and inner mitochondrial membranes. (A) Proteomic analysis of FLAG-tag immunoprecipitations as described in Materials and methods. For each on-bead digest of the indicated FLAG-tag purification or untagged wild type control (top row), the number of peptides (and percent coverage) are shown for each identified protein (left column). Data are represented as the mean ± standard error of three independent experiments. Asterisks indicate data represented as the mean ± standard deviation of two independent experiments. (B) Interdependent protein stability of components of the MitOS complex. The indicated relative amounts of whole-cell extract prepared from the indicated MitOS component FLAG-tag strain were subjected to SDS-PAGE and immunoblotting with α-FLAG, α-Ugo1, and α-G6PDH. (C) Genetic connection scatter plot of the average of MitOS component genes AIM5, AIM13, FCJ1, and AIM37. The x axis represents the cosine correlation between the mean of MitOS genes interaction scores, and the y axis indicates the mean interaction score between MitOS genes and each gene in the MITO-MAP. Every point in the scatter plot represents one gene. The cosine correlation values for points corresponding to the selected genes themselves were computed using the mean of the interaction score vectors for the remaining selected genes. In cases where the genetic interaction score was not measured, the point is plotted in gray along the line y = 0. (D) Genetic interaction scatter plot of the average of genes encoding components of MitOS (x axis) and POR1 (y axis). The x axis represents the mean of the genetic interaction scores of AIM5, AIM13, FCJ1, and AIM37 with each gene in the MITO-MAP, and the y axis indicates the mean interaction score of POR1 and each gene in the MITO-MAP. Significant common negative genetic interactions are highlighted. (E) Proteomic analysis of FLAG-tag immune purifications from cross-linked mitochondria as described in Materials and methods. For each on-bead digest of the indicated FLAG-tag protein or untagged wild-type control (top row), the number of peptides and percent coverage are shown for each identified protein (left column). Data are expressed as the mean ± standard error of three independent experiments.
Figure 3.

MitOS interacts with both outer and inner mitochondrial membranes. (A) Proteomic analysis of FLAG-tag immunoprecipitations as described in Materials and methods. For each on-bead digest of the indicated FLAG-tag purification or untagged wild type control (top row), the number of peptides (and percent coverage) are shown for each identified protein (left column). Data are represented as the mean ± standard error of three independent experiments. Asterisks indicate data represented as the mean ± standard deviation of two independent experiments. (B) Interdependent protein stability of components of the MitOS complex. The indicated relative amounts of whole-cell extract prepared from the indicated MitOS component FLAG-tag strain were subjected to SDS-PAGE and immunoblotting with α-FLAG, α-Ugo1, and α-G6PDH. (C) Genetic connection scatter plot of the average of MitOS component genes AIM5, AIM13, FCJ1, and AIM37. The x axis represents the cosine correlation between the mean of MitOS genes interaction scores, and the y axis indicates the mean interaction score between MitOS genes and each gene in the MITO-MAP. Every point in the scatter plot represents one gene. The cosine correlation values for points corresponding to the selected genes themselves were computed using the mean of the interaction score vectors for the remaining selected genes. In cases where the genetic interaction score was not measured, the point is plotted in gray along the line y = 0. (D) Genetic interaction scatter plot of the average of genes encoding components of MitOS (x axis) and POR1 (y axis). The x axis represents the mean of the genetic interaction scores of AIM5, AIM13, FCJ1, and AIM37 with each gene in the MITO-MAP, and the y axis indicates the mean interaction score of POR1 and each gene in the MITO-MAP. Significant common negative genetic interactions are highlighted. (E) Proteomic analysis of FLAG-tag immune purifications from cross-linked mitochondria as described in Materials and methods. For each on-bead digest of the indicated FLAG-tag protein or untagged wild-type control (top row), the number of peptides and percent coverage are shown for each identified protein (left column). Data are expressed as the mean ± standard error of three independent experiments.

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