Generation of the MITO-MAP. (A) Manually defined functional annotations for genes included in the MITO-MAP are summarized in pie charts: 592 genes whose products are predicted to localize to the mitochondria, 437 genes whose products are predicted to localize to the early secretory pathway (ESP), and 529 other genes. Localization groups were based on systematic results with GFP-tagged proteins and GO Slim component annotations. (B) Scatter plot of all full biological replicate genetic interaction score (S-score) measurements for double mutants generated from each of the two possible query-array parent strain combinations. The Pearson correlation for these replicates was 0.65.