Figure 4. Therapeutic Foxp3-directed T reg cell depletion fails to synergize with Gvax/αCTLA-4 in rejection of established tumors. (A) Foxp3-DTR transgenic mice or littermate WT control were injected with 900 ng of DT on days −1 and 0 or 8 and 9, challenged with B16/BL6 at day 0, and treated with Gvax/anti–CTLA-4 on days 8, 11, and 14. (B) Representative plot for the expression of CD25 and Foxp3+ by the CD4+ T cell compartment on days 2 and 4 after DT injection. (C) Tumor growth was monitored over time in Foxp3-DTR or littermate mice challenged with B16 melanoma and treated with Gvax/αCTLA-4 on days 8, 11, and 14. Foxp3-DTR mice were also treated with DT early (blue triangles; n = 10) or late (red squares; n = 10), as described in A. Littermate controls were also treated with DT early (black triangle; n = 10) or late (black square; n = 10). Data are representative of 3 independent experiments (n = 10 mice per group).
Figure 4.

Therapeutic Foxp3-directed T reg cell depletion fails to synergize with Gvax/αCTLA-4 in rejection of established tumors. (A) Foxp3-DTR transgenic mice or littermate WT control were injected with 900 ng of DT on days −1 and 0 or 8 and 9, challenged with B16/BL6 at day 0, and treated with Gvax/anti–CTLA-4 on days 8, 11, and 14. (B) Representative plot for the expression of CD25 and Foxp3+ by the CD4+ T cell compartment on days 2 and 4 after DT injection. (C) Tumor growth was monitored over time in Foxp3-DTR or littermate mice challenged with B16 melanoma and treated with Gvax/αCTLA-4 on days 8, 11, and 14. Foxp3-DTR mice were also treated with DT early (blue triangles; n = 10) or late (red squares; n = 10), as described in A. Littermate controls were also treated with DT early (black triangle; n = 10) or late (black square; n = 10). Data are representative of 3 independent experiments (n = 10 mice per group).

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