Figure 2. CD25 depletion prevents Gvax/αCTLA-4–induced T reg cell accumulation. Mice were injected with anti-CD25 mAb 4 d before or after challenge with B16/BL6, and then treated or not with Gvax or Gvax/αCTLA-4 on days 8 and 11, as shown in Fig. 1 A. LNs were analyzed for expression of CD4, Foxp3+, and KI-67 14 d after tumor challenge. Data in A represent the percentage of CD4+Foxp3+ cells obtained from individual mice. (B) The percentage of CD4+Foxp3+ T cells was multiplied by the total number of lymphocytes to calculate the absolute number of CD4+Foxp3+ T reg cells. Data in C represent the percentage of KI-67+ cells within the CD4+Foxp3+ compartment. Closed circles represent no depletion, and open circles represent CD25 depletion. Data are representative of 3 independent experiments (n = 3 mice per group).
Figure 2.

CD25 depletion prevents Gvax/αCTLA-4–induced T reg cell accumulation. Mice were injected with anti-CD25 mAb 4 d before or after challenge with B16/BL6, and then treated or not with Gvax or Gvax/αCTLA-4 on days 8 and 11, as shown in Fig. 1 A. LNs were analyzed for expression of CD4, Foxp3+, and KI-67 14 d after tumor challenge. Data in A represent the percentage of CD4+Foxp3+ cells obtained from individual mice. (B) The percentage of CD4+Foxp3+ T cells was multiplied by the total number of lymphocytes to calculate the absolute number of CD4+Foxp3+ T reg cells. Data in C represent the percentage of KI-67+ cells within the CD4+Foxp3+ compartment. Closed circles represent no depletion, and open circles represent CD25 depletion. Data are representative of 3 independent experiments (n = 3 mice per group).

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