Figure 7. Sustained production of PI4,5P2 inhibits phagocytosis. (a) Schematic of the experimental protocol. The addition of rapamycin induces the heterodimerization of the YFP-FKBP-5K construct with a plasma membrane–targeted form of FRB. (b) Distribution of YFP-FKBP-5K and RFP-PH–PLC-δ before (−Rap) and 5 min after the addition of 10 µM rapamycin (+Rap). Arrowhead identifies sealed and internalized phagosomes, and the arrow highlights forming phagosomes. (c) Internalization and adherence of beads to macrophages was quantified in cells expressing YFP or YFP-FKBP-5K and plasma membrane–targeted FRB with or without the addition of rapamycin. Data are means ± SEM of at least 500 beads per condition.
Figure 7.

Sustained production of PI4,5P2 inhibits phagocytosis. (a) Schematic of the experimental protocol. The addition of rapamycin induces the heterodimerization of the YFP-FKBP-5K construct with a plasma membrane–targeted form of FRB. (b) Distribution of YFP-FKBP-5K and RFP-PH–PLC-δ before (−Rap) and 5 min after the addition of 10 µM rapamycin (+Rap). Arrowhead identifies sealed and internalized phagosomes, and the arrow highlights forming phagosomes. (c) Internalization and adherence of beads to macrophages was quantified in cells expressing YFP or YFP-FKBP-5K and plasma membrane–targeted FRB with or without the addition of rapamycin. Data are means ± SEM of at least 500 beads per condition.

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