Figure 6. Redistribution of PIP5Ks during phagocytosis. (a) Macrophages were cotransfected with the specified GFP-PIP5K isoform and PM-RFP. Phagocytosis was initiated by exposure to IgG-opsonized beads, and the cells were imaged by spinning-disc microscopy. Arrowheads indicate sealed and internalized phagosomes, and arrows indicate forming phagosomes. The representative images shown were acquired 180 s after initiation of phagocytosis. (b) Quantification of the fluorescence intensity of the phagosomal membrane relative to the plasmalemma for each of the PIP5K isoforms and for PM-RFP. Data are means ± SEM (n ≥ 30). (c) Cells were cotransfected with the indicated constructs and treated with 100 nM wortmannin for 10 min before initiation of phagocytosis. The representative images shown were acquired 180 s after initiation of phagocytosis. Bars, 3 µm.
Figure 6.

Redistribution of PIP5Ks during phagocytosis. (a) Macrophages were cotransfected with the specified GFP-PIP5K isoform and PM-RFP. Phagocytosis was initiated by exposure to IgG-opsonized beads, and the cells were imaged by spinning-disc microscopy. Arrowheads indicate sealed and internalized phagosomes, and arrows indicate forming phagosomes. The representative images shown were acquired 180 s after initiation of phagocytosis. (b) Quantification of the fluorescence intensity of the phagosomal membrane relative to the plasmalemma for each of the PIP5K isoforms and for PM-RFP. Data are means ± SEM (n ≥ 30). (c) Cells were cotransfected with the indicated constructs and treated with 100 nM wortmannin for 10 min before initiation of phagocytosis. The representative images shown were acquired 180 s after initiation of phagocytosis. Bars, 3 µm.

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