Figure 5. The localization of PIP5Ks is altered by depressing the surface charge. (a and b) Cells were cotransfected with the specified GFP-PIP5K isoform and RFP-PH–PLC-δ (insets). Images were acquired before and after incubation with either 2-deoxy-glucose plus antimycin for 40 min (a) or with 10 µM ionomycin for 5 min (b). (c) Quantification of the membrane association of GFP-PIP5K isoforms and of PH–PLC-δ before and after the treatments described in a and b. Data are means ± SEM (n ≥ 30). Bars, 3 µm.
Figure 5.

The localization of PIP5Ks is altered by depressing the surface charge. (a and b) Cells were cotransfected with the specified GFP-PIP5K isoform and RFP-PH–PLC-δ (insets). Images were acquired before and after incubation with either 2-deoxy-glucose plus antimycin for 40 min (a) or with 10 µM ionomycin for 5 min (b). (c) Quantification of the membrane association of GFP-PIP5K isoforms and of PH–PLC-δ before and after the treatments described in a and b. Data are means ± SEM (n ≥ 30). Bars, 3 µm.

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