Figure 1. Expression and localization of PIP5Ks in RAW cells. (a) Immunoblots of RAW cell and brain extracts probed with PIP5K isoform-specific antibodies. (b and c) Colocalization of PIP5K isoforms with their product, PI4,5P2, at the plasma membrane. RAW cells were transiently cotransfected with GFP or YFP chimeras of the PIP5K isoforms and with either RFP-PH–PLC-δ (b) or PM-RFP used as a plasmalemmal marker (c). The distribution of the fluorescent proteins was analyzed by spinning-disc confocal microscopy, and representative images acquired near the middle of the cell are illustrated. Bars, 3 µm.
Figure 1.

Expression and localization of PIP5Ks in RAW cells. (a) Immunoblots of RAW cell and brain extracts probed with PIP5K isoform-specific antibodies. (b and c) Colocalization of PIP5K isoforms with their product, PI4,5P2, at the plasma membrane. RAW cells were transiently cotransfected with GFP or YFP chimeras of the PIP5K isoforms and with either RFP-PH–PLC-δ (b) or PM-RFP used as a plasmalemmal marker (c). The distribution of the fluorescent proteins was analyzed by spinning-disc confocal microscopy, and representative images acquired near the middle of the cell are illustrated. Bars, 3 µm.

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