Myo2p point mutants defective in peroxisome inheritance are not impaired in vacuolar or mitochondrial inheritance. Cells expressing wild-type MYO2 or mutant myo2 were grown in SCIM to induce peroxisome formation, and confocal images were captured. (A and B) Peroxisomes were labeled with Pot1p-GFP, mitochondria were labeled with MitoTracker red (A), and vacuoles were labeled with FM4-64 (B). Quantification of mitochondria and vacuole inheritance was performed as in Fig. 2 B. Graphic results show the means ± SEM of three independent experiments. Bars, 5 µm. (C) Growth of cells expressing wild-type MYO2 or mutant myo2 on glucose-containing YPD medium. Cells were grown to mid-log phase in liquid YPD, and equal amounts of cells were serially diluted 10-fold onto YPD agar and incubated at 23 or 30°C. (D) Surface representation of the Myo2p globular tail, indicating the regions that bind peroxisomes (yellow) and secretory vesicles (teal). Subdomains I and II are shown in blue and red, respectively.