Stress response transcription factor Gis1 is required for life span extension and attenuated genomic instability associated with SCH9 deficiency. (A) Chronological survival (n = 11) and (B) mutation frequency in the CAN1 gene (n = 11) of the wild type (WT; DBY746) and sch9Δ mutants. ^, P < 0.05; ^^, P < 0.01; two-tailed t test, sch9Δ gis1Δ versus sch9Δ at the indicated time points. (C) mRNA levels of REV1, REV3, REV7, and recombination genes involved in DNA repair. WT (DBY746) and sch9Δ were grown in SDC media. Cells were collected on day 2.5 for mRNA extraction and microarray analysis. Data represent mean ± SEM (error bars) of percentage change, sch9Δ versus WT (n = 3). For complete microarray data, see Table S2.