Figure 5. Keratins regulate AMPK activity through localization of GLUT1 and -3. (A and B) Double immunofluorescence of keratins and GLUT1 and -3 (A and B) in WT yolk sac tissue. (A′, A″, B′, and B″) Yolk sacs of E9.5 WT and KtyII−/− embryos were analyzed for GLUT1 (A′ and A″) and -3 (B′ and B″) localization. Insets show enlargement of the region encompassing the plasma membrane. (C) mRNA expression of GLUT transporters in E9.5 WT and mutants. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a normalization control. (D) Total GLUT1 and -3 protein levels in protein lysates of E9.5 keratin WT and KtyII−/− embryos. Error bars represent SEM. G, GLUT; T, tubulin. (E) Model for function of apical keratins in GLUT localization. Our model suggests that full activation of mTORC1 depends on the correct localization of GLUT1 and -3 by subapical keratins in the mouse embryo. Bars, 10 µm.
Figure 5.

Keratins regulate AMPK activity through localization of GLUT1 and -3. (A and B) Double immunofluorescence of keratins and GLUT1 and -3 (A and B) in WT yolk sac tissue. (A′, A″, B′, and B″) Yolk sacs of E9.5 WT and KtyII−/− embryos were analyzed for GLUT1 (A′ and A″) and -3 (B′ and B″) localization. Insets show enlargement of the region encompassing the plasma membrane. (C) mRNA expression of GLUT transporters in E9.5 WT and mutants. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a normalization control. (D) Total GLUT1 and -3 protein levels in protein lysates of E9.5 keratin WT and KtyII−/− embryos. Error bars represent SEM. G, GLUT; T, tubulin. (E) Model for function of apical keratins in GLUT localization. Our model suggests that full activation of mTORC1 depends on the correct localization of GLUT1 and -3 by subapical keratins in the mouse embryo. Bars, 10 µm.

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