Geminin injection promotes premature chromatin condensation and mitosis with unreplicated chromatids. Frames showing nuclei from embryos expressing Histone-RFP and Cid-GFP in the cycle after injection with control buffer (A) or Geminin (B). In the Geminin-injected embryo, chromatin begins to condense ∼3 min into interphase, kinetochores are unpaired in mitosis, and chromosomes fail to congress to the metaphase plate. Time is presented as min:sec. (C) Higher magnification view of Cid-GFP during mitosis in control and Geminin injections. Bifurcated arrows in the control nucleus point to paired kinetochores at metaphase. Single arrows in Geminin injection highlight unpaired kinetochores.