Figure 8. The ubiquitin conjugation system is involved in a signal transduction pathway in flagella. (A and B) fla3 cells were shifted to 32°C for 1 and 2 h, and immunoblots of flagellar proteins were probed with antibodies against PKD2 (A) and CrPKG (B). Arrows point to putative ubiquitinated forms of PKD2 and CrPKG. (C) The flagella of plus and minus gametes before (0 min) and after mating for 5 min were isolated and probed with ubiquitin antibody. The amount of ubiquitinated proteins increased after 5 min of mating. β-Tubulin was used as a loading control. (D) Flagella were isolated from mating-type minus and plus gametes expressing CrUbc13∷GFP before (0 min) and after mating for 5 min. Flagellar proteins (20, 10, and 5 μg) were probed for GFP. The amount of the CrUbc13∷GFP increased quickly during mating. β-Tubulin was used as a loading control. Molecular masses are given in kilodaltons.
Figure 8.

The ubiquitin conjugation system is involved in a signal transduction pathway in flagella. (A and B) fla3 cells were shifted to 32°C for 1 and 2 h, and immunoblots of flagellar proteins were probed with antibodies against PKD2 (A) and CrPKG (B). Arrows point to putative ubiquitinated forms of PKD2 and CrPKG. (C) The flagella of plus and minus gametes before (0 min) and after mating for 5 min were isolated and probed with ubiquitin antibody. The amount of ubiquitinated proteins increased after 5 min of mating. β-Tubulin was used as a loading control. (D) Flagella were isolated from mating-type minus and plus gametes expressing CrUbc13∷GFP before (0 min) and after mating for 5 min. Flagellar proteins (20, 10, and 5 μg) were probed for GFP. The amount of the CrUbc13∷GFP increased quickly during mating. β-Tubulin was used as a loading control. Molecular masses are given in kilodaltons.

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