Ubiquitinated proteins increase during flagellar resorption. (A–C) Flagella were isolated at the indicated times (NDF, not deflagellated) during regeneration (Reg; A) or resorption (Res) induced by high Na⁺/no-Ca²⁺ medium (B) or mating (C). Immunoblots were probed with ubiquitin (Ub) antibody. β-Tubulin or IFT139 was used as a loading control. The mean length of flagella is shown beneath each lane. Molecular masses are given in kilodaltons.