Figure 2. CrUbc13 is present in the flagella of C. reinhardtii. (A) Isolated flagella (F), membrane/matrix (M), and axonemal (Ax) fractions from cells expressing CrUbc13∷GFP were analyzed on immunoblots probed with antibodies to GFP, FMG-1 (the major membrane glycoprotein in the flagella), and RSP3. FMG-1 and RSP3 were used to monitor the purity of the membrane/matrix and axonemal fractions, respectively. Molecular masses are given in kilodaltons. (B) The box in this differential interference contrast picture of a cell that expresses CrUbc13∷GFP delineates the region illuminated with a 488-nm laser in C. (C) Fluorescence from the illuminated area (box) demonstrates that CrUbc13∷GFP is present in the flagellum. Although only the distal two thirds of the flagella were irradiated, there is some spillover of the light, causing the cell body region to emit fluorescence. Bars, 5 μm.
Figure 2.

CrUbc13 is present in the flagella of C. reinhardtii. (A) Isolated flagella (F), membrane/matrix (M), and axonemal (Ax) fractions from cells expressing CrUbc13∷GFP were analyzed on immunoblots probed with antibodies to GFP, FMG-1 (the major membrane glycoprotein in the flagella), and RSP3. FMG-1 and RSP3 were used to monitor the purity of the membrane/matrix and axonemal fractions, respectively. Molecular masses are given in kilodaltons. (B) The box in this differential interference contrast picture of a cell that expresses CrUbc13∷GFP delineates the region illuminated with a 488-nm laser in C. (C) Fluorescence from the illuminated area (box) demonstrates that CrUbc13∷GFP is present in the flagellum. Although only the distal two thirds of the flagella were irradiated, there is some spillover of the light, causing the cell body region to emit fluorescence. Bars, 5 μm.

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