Figure 1. Free ubiquitin and ubiquitinated proteins are present in the flagella of C. reinhardtii. (A) Isolated flagella (F) were extracted with the indicated concentration of NP-40, and the soluble membrane/matrix (M) and insoluble axonemal (Ax) fractions were analyzed on immunoblots probed with an antibody to ubiquitin (Ub; P4G7; Covance). A Coomassie blue–stained gel (CB) of the loaded proteins is shown at the bottom. Monomeric ubiquitin is 7 kD. (B) Immunoblot analysis shows that HA∷ubiquitin is present in the flagella and membrane/matrix of the strain CRH21, which harbors the HA∷ubiquitin transgene. No signal of HA∷ubiquitin was present in wild-type (WT) flagella. A Coomassie blue–stained gel of the loaded proteins is shown at the bottom. Mobilities of prestained molecular mass standards are indicated in kilodaltons.
Figure 1.

Free ubiquitin and ubiquitinated proteins are present in the flagella of C. reinhardtii. (A) Isolated flagella (F) were extracted with the indicated concentration of NP-40, and the soluble membrane/matrix (M) and insoluble axonemal (Ax) fractions were analyzed on immunoblots probed with an antibody to ubiquitin (Ub; P4G7; Covance). A Coomassie blue–stained gel (CB) of the loaded proteins is shown at the bottom. Monomeric ubiquitin is 7 kD. (B) Immunoblot analysis shows that HA∷ubiquitin is present in the flagella and membrane/matrix of the strain CRH21, which harbors the HA∷ubiquitin transgene. No signal of HA∷ubiquitin was present in wild-type (WT) flagella. A Coomassie blue–stained gel of the loaded proteins is shown at the bottom. Mobilities of prestained molecular mass standards are indicated in kilodaltons.

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