Figure 4.
Figure 4. MRT4 gain of function alleles can rescue the slow growth and 60S export defects of yvh1Δ cells. (A) YVH1 and yvh1Δ cells carrying the indicated plasmids were spotted in serial 10-fold dilutions onto SD-Leu plates and incubated at 25, 30, or 37°C for 3 d. (B) yvh1Δ cells containing the 60S subunit reporter L25-GFP were transformed with the indicated plasmids. The subcellular localization of L25-GFP was visualized by fluorescence microscopy. (C) Sequence alignments of RPP0 and MRT4 from the indicated organisms were performed using the program ClustalX. Asterisks indicate the conserved amino acids that make contact with 25S rRNA that have been subjected to site-directed mutagenesis. (D) Rescue of the slow-growth phenotype by mutations in 25S rRNA–interacting amino acid residues. yvh1Δ cells carrying the indicated plasmids were spotted in serial 10-fold dilutions onto SD-Leu plates and incubated at 30°C for 3 d.

MRT4 gain of function alleles can rescue the slow growth and 60S export defects of yvh1Δ cells. (A) YVH1 and yvh1Δ cells carrying the indicated plasmids were spotted in serial 10-fold dilutions onto SD-Leu plates and incubated at 25, 30, or 37°C for 3 d. (B) yvh1Δ cells containing the 60S subunit reporter L25-GFP were transformed with the indicated plasmids. The subcellular localization of L25-GFP was visualized by fluorescence microscopy. (C) Sequence alignments of RPP0 and MRT4 from the indicated organisms were performed using the program ClustalX. Asterisks indicate the conserved amino acids that make contact with 25S rRNA that have been subjected to site-directed mutagenesis. (D) Rescue of the slow-growth phenotype by mutations in 25S rRNA–interacting amino acid residues. yvh1Δ cells carrying the indicated plasmids were spotted in serial 10-fold dilutions onto SD-Leu plates and incubated at 30°C for 3 d.

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