![Figure 3. The half-life of AID differs according to its subcellular localization. (A and B) BL2 clones expressing WT-AID-EGFP, MutNES-AID-EGFP, or MutNLS-AID-EGFP were metabolically labeled for 1 h with [35S]-labeled cysteine and methionine and chased for the indicated length of time. AID-EGFP was immunoprecipitated from cell lysates with anti-EGFP antibody (or anti-AID for the endogenous protein) and subjected to SDS-PAGE and autoradiography. (C) Immunoprecipitated MutNES-AID-EGFP was transferred into nitrocellulose and probed with a monoclonal HRP-conjugated anti-EGFP antibody to control for immunoprecipitation efficiency. (D) Densitometry analysis of autoradiographs of pulse-chase immunoprecipitated products, expressed as percentage of initial labeling. The data shown are the mean of the results from two experiments.](https://cdn.rupress.org/rup/content_public/journal/jem/205/6/10.1084_jem.20070950/7/jem_20070950_gs_fig3.jpeg?Expires=1767749333&Signature=eA~qWZh65Yw9QB9KwYqXbdP-nvKlp~uuZLZzZeEzIdeGFb7WP3rHuGmi26yfoJu15r4mm5XbOPbAP7viSJpBqZiWnJ4eHWEpyF2zLNIqLSU3KJ4lfrAwZTxLz65Qt9qoQH2UDbgDNa2qoKmyfaGkXM1lED8iLD7LAhP~M6JHkZAY2ugehDbcMKdy6xMFDweTLqS8kjxN~ldfOadPvgVhXOYCOX2pNbjg6s3Zj6iUPtbW~1p-~PnF6206Pk0RNJuBiTBB5POtpeD5xAQ4aCg4~WSBRtr4kLQ5PXcxLMRFFVCddPG6qBafUgs3cglRfjotzhL5f8ZClDoSIq2oO7frtg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The half-life of AID differs according to its subcellular localization. (A and B) BL2 clones expressing WT-AID-EGFP, MutNES-AID-EGFP, or MutNLS-AID-EGFP were metabolically labeled for 1 h with [35S]-labeled cysteine and methionine and chased for the indicated length of time. AID-EGFP was immunoprecipitated from cell lysates with anti-EGFP antibody (or anti-AID for the endogenous protein) and subjected to SDS-PAGE and autoradiography. (C) Immunoprecipitated MutNES-AID-EGFP was transferred into nitrocellulose and probed with a monoclonal HRP-conjugated anti-EGFP antibody to control for immunoprecipitation efficiency. (D) Densitometry analysis of autoradiographs of pulse-chase immunoprecipitated products, expressed as percentage of initial labeling. The data shown are the mean of the results from two experiments.
