Ectopic twist1 overexpression controls DTH. (A) Naive DO11.10 Th cells were stimulated under Th1-polarizing conditions. On d 2, cells were infected with control virus (circles) or twist1-encoding virus (squares). On d 6, infected GFP⁺ cells were injected i.v. into BALB/c mice. The DTH response was induced by s.c. OVA_323-339/IFA injection into the left footpad (filled symbols), and Δ footpad thickness (mean ± SD; n = 4, Mann-Whitney test, nonparametric) was determined thereafter. Injection of PBS/IFA served as control (open symbols). (B) Ex vivo IFN-γ mRNA expression in transferred GFP⁺ Th1 cells 24 h after DTH induction isolated from the draining popliteal lymph node (left foot). (C) To monitor the migratory capacity of the transferred cell populations, infected GFP⁺ Th cells were radiolabeled and injected i.v. into BALB/c mice 1 d after induction of the DTH response. 24 h later radioactivity recovered from indicated tissues was determined using a γ-counter (mean ± SD; n = 4).