Genes differentially expressed upon ectopic twist1 overexpression. Splenic DO11.10 cells were activated in vitro with the cognate peptide OVA327-339 in the presence of 1 ng/ml IL-12 and 1 ng/ml IL-2. On d 2, cells were infected with control retrovirus, or twist1-encoding virus. On d 5, cells were sorted according to expression of the viral marker gene gfp. Cells were restimulated for 4 h with PMA/ionomycin. The transcriptional profiles of duplicates of cultures were compared.