Figure 3. Distribution of PS during phagosome formation and maturation. (A–C) RAW macrophages cotransfected with mRFP-Palm and the PS biosensor GFP-Lact-C2. The cells were exposed to IgG-opsonized sRBC. Confocal images were acquired 1 min (A), 5 min (B), or 1 h (C) after initiation of phagocytosis. The GFP fluorescence of the PS biosensor is shown in the left column, mRFP-Palm is shown in the middle, and the two channels were merged in the right column. Images in A–C are representative of 30 cells from two independent experiments. (D) Quantification of the fluorescence intensity of GFP-Lact-C2 (green bars) and mRFP-Palm (red bars) at the specified stage of maturation. The results are presented as the ratio of fluorescence intensity of the phagosome to that in the bulk, unengaged plasma membrane (PM). Asterisks indicate location of sRBCs. Data are means ± SD of 10 cells from a typical experiment. Bars, 2 µm.
Figure 3.

Distribution of PS during phagosome formation and maturation. (A–C) RAW macrophages cotransfected with mRFP-Palm and the PS biosensor GFP-Lact-C2. The cells were exposed to IgG-opsonized sRBC. Confocal images were acquired 1 min (A), 5 min (B), or 1 h (C) after initiation of phagocytosis. The GFP fluorescence of the PS biosensor is shown in the left column, mRFP-Palm is shown in the middle, and the two channels were merged in the right column. Images in A–C are representative of 30 cells from two independent experiments. (D) Quantification of the fluorescence intensity of GFP-Lact-C2 (green bars) and mRFP-Palm (red bars) at the specified stage of maturation. The results are presented as the ratio of fluorescence intensity of the phagosome to that in the bulk, unengaged plasma membrane (PM). Asterisks indicate location of sRBCs. Data are means ± SD of 10 cells from a typical experiment. Bars, 2 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal