DE-cad and Arm form puncta between apical surface microvilli. (A–C) Deconvolved images over cellularization are shown. (top) 3D views of single cells shown with front and back removed. (bottom) Projections of apical surface, apicolateral, and BJs. Arrows indicate surface puncta, and brackets indicate SAJs. (D) Mobile DE-cad∷GFP apical surface puncta (arrow). (E) DE-cad∷GFP (green) and tubulin∷mCherry (red) mid cellularization is shown. (F–G) DE-cad∷GFP (green) and actin∷RFP (red) are shown. Images are deconvolved. Early (F) and mid (G) cellularization are shown. Single plane side and surface views are shown. Insets show DE-cad∷GFP puncta between microvilli. (H) Latrunculin A effect on apical surface DE-cad∷GFP/actin∷RFP shown with dual imaging. Images are deconvolved. Experiment was performed at early cellularization. Bars, 5 µm.