Figure 4. The high susceptibility of Syn4KO mice to ConA-induced hepatic injury. (A) The plasma levels of ALT and AST in wild-type (Cont) and Syn4KO mice at 12 h after ConA injection (n = 6 per each group). (B) Representative histology of liver sections stained by H-E 12 h after ConA injection, and the survival curve after ConA injection (n = 8 and 13 wild type and Syn4KO, respectively). Dotted lines indicate the area of liver degeneration. The degenerative area per liver section was quantified by using ImageJ (n = 3 per each group). Bar, 200 μm. (C) The plasma levels of full-length and thrombin-cleaved OPN in wild-type (Cont) and Syn4KO mice at 12 h after ConA injection (n = 4 and 6 wild type and Syn4KO, respectively). (D) The attenuation of ConA-induced hepatic injury in Syn4KO mice by anti-OPN (M5) antibody treatment (n = 5 per group). Syn4KO mice were intraperitoneally treated with M5 antibody and control normal rabbit IgG (Cont Ig; 400 μg/head) at 15 h before ConA injection. Plasma ALT levels and representative histology (HE) are shown. Degenerative area per liver section was quantified by using ImageJ (n = 5 per each group). Data are presented as means ± SEM. *, P < 0.05; **, P < 0.005. Bar, 500 μm.
Figure 4.

The high susceptibility of Syn4KO mice to ConA-induced hepatic injury. (A) The plasma levels of ALT and AST in wild-type (Cont) and Syn4KO mice at 12 h after ConA injection (n = 6 per each group). (B) Representative histology of liver sections stained by H-E 12 h after ConA injection, and the survival curve after ConA injection (n = 8 and 13 wild type and Syn4KO, respectively). Dotted lines indicate the area of liver degeneration. The degenerative area per liver section was quantified by using ImageJ (n = 3 per each group). Bar, 200 μm. (C) The plasma levels of full-length and thrombin-cleaved OPN in wild-type (Cont) and Syn4KO mice at 12 h after ConA injection (n = 4 and 6 wild type and Syn4KO, respectively). (D) The attenuation of ConA-induced hepatic injury in Syn4KO mice by anti-OPN (M5) antibody treatment (n = 5 per group). Syn4KO mice were intraperitoneally treated with M5 antibody and control normal rabbit IgG (Cont Ig; 400 μg/head) at 15 h before ConA injection. Plasma ALT levels and representative histology (HE) are shown. Degenerative area per liver section was quantified by using ImageJ (n = 5 per each group). Data are presented as means ± SEM. *, P < 0.05; **, P < 0.005. Bar, 500 μm.

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