Figure 8. Loss of Bmf enhances γ... | Rockefeller University Press

Figure 8.

$Figure 8. Loss of Bmf enhances γ irradiation induced thymic lymphoma development. Mice of the indicated genotypes (7–8 wk of age) were exposed to whole body irradiation using 2.5 Gy. Animals were killed 20 h later, and thymocyte numbers were evaluated by FACS analysis of cell surface marker expression (A) and cell counting to calculate the total number of thymocytes (B) and CD4+8+ immature thymocytes (C). Bars represent means ± the SEM of 3 WT and 5 Bmf-deficient animals and two independent experiments. Significant differences in depletion of total thymocytes (P = 0.0044) and CD4+8+ thymocytes (P = 0.003) were confirmed using ANOVA analysis. Cohorts of WT (n = 16), bmf−/− (n = 8), and p53+/− (n = 9) mice were exposed to a fractionated γ irradiation protocol (4 × 1.75 Gy in weekly intervals, starting at 4 wk of age) and monitored for the development of thymic lymphomas over time. (B) Kaplan-Meier analysis of tumor-free survival of mice of the indicated genotypes. Log rank (Mantle-Cox) analysis was used to calculate differences between WT and p53+/− (P = 0.027) and WT and bmf−/− mice (P = 0.009).$

Loss of Bmf enhances γ irradiation induced thymic lymphoma development. Mice of the indicated genotypes (7–8 wk of age) were exposed to whole body irradiation using 2.5 Gy. Animals were killed 20 h later, and thymocyte numbers were evaluated by FACS analysis of cell surface marker expression (A) and cell counting to calculate the total number of thymocytes (B) and CD4⁺8⁺ immature thymocytes (C). Bars represent means ± the SEM of 3 WT and 5 Bmf-deficient animals and two independent experiments. Significant differences in depletion of total thymocytes (P = 0.0044) and CD4⁺8⁺ thymocytes (P = 0.003) were confirmed using ANOVA analysis. Cohorts of WT (n = 16), bmf^−/− (n = 8), and p53^+/− (n = 9) mice were exposed to a fractionated γ irradiation protocol (4 × 1.75 Gy in weekly intervals, starting at 4 wk of age) and monitored for the development of thymic lymphomas over time. (B) Kaplan-Meier analysis of tumor-free survival of mice of the indicated genotypes. Log rank (Mantle-Cox) analysis was used to calculate differences between WT and p53^+/− (P = 0.027) and WT and bmf^−/− mice (P = 0.009).