A Mir-284 transgene rescues GluR phenotypes caused by deletion of the native MiR-284 gene. (A) Larvae hemizygous for each of two different MiR-284 deletions (Df(3R)kar-Sz12/Df(3R)exel7317) show abundant mislocalized GluRIIA and GluRIIB in body wall muscles (left), and this phenotype is largely rescued with a single copy of a MiR-284 transgene (right). (B and C) Quantification of synaptic (B) and nonsynaptic (C) GluRIIA and GluRIIB immunoreactivity (green and red, respectively) for homozygous Df(3R)kar-Sz12 mutants, mutants of the genotype Df(3R)kar-Sz12/Df(3R)exel7317, and each of these two mutants expressing a MiR-284 transgene. Error bars represent SEM. n.s., not significant. ***, P < 0.0001. (D) Pie charts showing relative amounts of GluRIIA and GluRIIB immunoreactivity in control NMJs, in NMJs after deletion of MiR-284, and in MiR-284 deletion mutants rescued with a MiR-284 transgene.