Figure 8.
Figure 8. Genetic deletion of MiR-284 changes synaptic GluR subunit composition and leads to accumulation of nonsynaptic GluRIIA and GluRIIB protein. (A) Type and general location of microRNA-binding sites in GluRIIA, GluRIIB, and GluRIIC mRNAs, based on refined bioinformatics predictions (See main text and Tables S1–S3). (B) Larvae homozygous for a deletion (Df(3R)kar-Sz12) that removes MiR-284 show abundant mislocalized GluRIIA and GluRIIB in body wall muscles. (C) Quantification from images like that shown in B. GluRIIB protein is dramatically increased both in synapses and in nonsynaptic (intracellular) areas, whereas GluRIIA is increased only nonsynaptically (n = 10–12). ***, P < 0.0001. (D) GluRIIC is not changed in MiR-284 deletion mutants (Df(3R)kar-Sz12 ; n = 5). Error bars represent SEM. n.s., not significant.

Genetic deletion of MiR-284 changes synaptic GluR subunit composition and leads to accumulation of nonsynaptic GluRIIA and GluRIIB protein. (A) Type and general location of microRNA-binding sites in GluRIIA, GluRIIB, and GluRIIC mRNAs, based on refined bioinformatics predictions (See main text and Tables S1–S3). (B) Larvae homozygous for a deletion (Df(3R)kar-Sz12) that removes MiR-284 show abundant mislocalized GluRIIA and GluRIIB in body wall muscles. (C) Quantification from images like that shown in B. GluRIIB protein is dramatically increased both in synapses and in nonsynaptic (intracellular) areas, whereas GluRIIA is increased only nonsynaptically (n = 10–12). ***, P < 0.0001. (D) GluRIIC is not changed in MiR-284 deletion mutants (Df(3R)kar-Sz12 ; n = 5). Error bars represent SEM. n.s., not significant.

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