NMJ synaptic transmission is largely unaltered after muscle-specific knockdown of dicer-1 RNAi. Synaptic transmission was assayed by voltage-clamping postsynaptic ventral longitudinal muscle 6 (at −60 mV) and recording either evoked (EJC) or spontaneous (sEJC) synaptic transmission at NMJs. (A) EJC amplitude is not significantly changed after muscle-specific knockdown of dicer-1 (n = 8–10 for each genotype). (B) Knockdown of muscle dicer-1 also does not significantly change sEJC (“mini”) frequency (n = 10–20 animals of each genotype; thousands of sEJCs). (C and D) Muscle-specific knockdown of dicer-1 leads to a slight increase in sEJC amplitude, which suggests a slight increase in the number of functional postsynaptic GluRs (n = 10–20 animals of each genotype; thousands of sEJCs). Error bars represent SEM. n.s., not significant. *, P < 0.01.