Dicer-1 knockdown leads to an increase in GluR mRNA. (A) Real-time RT-PCR shows that GluRIIA and GluRIIB mRNA is increased after expression of dicer-1 RNAi in postsynaptic muscles (24BGal4/UAS-dcr1.RNAi), compared with wild-type controls (n = 9–10 of each genotype). (B) FISH shows a similar increase in GluRIIA mRNA in postsynaptic muscles, as measured by density of visible GluRIIA mRNA aggregates (n = 6 of each genotype). Error bars represent SEM. *, P < 0.01; **, P < 0.001. (C) Representative FISH images of NMJs on muscles 6 and 7 (as in Fig. 1 B) from control animals and after postsynaptic expression of dicer-1 (24BGal4/UAS-dcr1.RNAi). (D) Model explaining how expression of dicer-1 RNAi in body wall muscles could lead to an increase in GluR protein, assuming microRNAs regulate GluR subunit mRNAs.