Phenotypic changes in antigen-specific CD8⁺ T cells after combinatorial therapeutic vaccination. (A) Expression of granzyme B on D^bGP33-41 tetramer-positive cells in PBMC at the indicated times after therapy and on D^bGP33-41 or D^bGP276-286 tetramer-positive cells in spleen at 4 wk after therapy. Open histograms, granzyme B; filled histograms, isotype control. Numbers represent mean fluorescence intensity of granzyme B expression. PD-1 expression on D^bGP33-41 or D^bGP276-286 tetramer-positive cells (B) and CD127 expression on D^bGP33-41 tetramer-positive cells (C) in different tissues at 4 wk after therapy. Numbers represent the frequency of tetramer-positive cells expressing CD127 or PD-1. The data are representative of two independent experiments.