Suppression of TIP47 affects LD maturation. (A and B) TIP47 (red) and neutral lipid (green) after oleic acid feeding. (C and D) Merge of the differential interference contrast image and the neutral lipid stain of the cells shown in A and B. Fluorescence intensities were quantified per cell as indicated by the red regions. Bar, 15 µm. (E) Flow cytofluorometry of living cells. Bars in the first column represent the background geometric mean fluorescence intensities of Bodipy 493/503 in cells at steady state. Bars in the second column display the Bodipy 493/503 fluorescence intensities after oleic acid feeding. Error bars represent the variation of nine independent experiments. (F) Time course of [¹⁴C]oleic acid uptake into cells. Error bars indicate the experimental variation between three experiments. (G) Verification of the TIP47 KD. Values on blot are shown in kilodaltons. (H) TLC of lipids from PNS and isolated LDs of control and TIP47 KD cells after oleic acid (OA) feeding. Chol, cholesterol; PE, phosphatidylethanolamine; PC, phosphatidylcholine.